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Methods of isotope ratio analysis
For the isotope ratio determinations, the samples are combusted by methods of the elemental analysis. The gases/ash (of metals) formed are/is separated and their/its isotope ratios are determined by means of isotope ratio mass spectrometry (IRMS). This procedure is preferably performed in the form of the multi-element isotope ratio analysis and leads to bulk δ-values of the samples. The data for the individual elements provide complementary information.
For defined chemical substances (aroma compounds, synthetic chemicals), the deuterium nuclear resonance spectrometry (2H-NMR) provides hydrogen isotopic patterns, containing quite detailed information on the compounds´ (bio)-syntheses. 13-C isotopic patterns of defined food components (ethanol, glycerol, ascorbic acid, malic acid) contain further essential information and can be obtained by defined degradation of the compounds.
Examples of products and questions to be solved
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